EGF Protéine

N° du produit ABIN7680472

Protéine Recombinant EGF exprimée dans Yeast (Saccharomyces Cerevisiae).

Aperçu rapide pour EGF Protéine (ABIN7680472)

Antigène: EGF (Epidermal Growth Factor (EGF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Yeast (Saccharomyces Cerevisiae)

Application: Cell Culture (CC)

Pureté: More than 95 % as determined by SDS-PAGE. 

Classe de qualité: Animal-Free

Détail du produit

Fonction: rhEGF - Recombinant human Epidermal growth factor

Attributs du produit: This product arrives as White lyophilized powder 

niveau d'endotoxine: Less than 1 EU/mg

Biological Activity Comment: The ED50 is ≤1ng/ml determined by a cell proliferation assay using Balb/c 3T3 cell, corresponding to a specific activity of ≥1x10^6 Units/mg.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is recommended to reconstitute the lyophilized OsrhEGF in sterile water.

Buffer: It is lyophilized with Mannitol as the stabilizer.

Conseil sur la manipulation: Use as soon as possible after opening. Avoid freeze-thaw cycles

Stock: -20 °C

Stockage commentaire: Stored at temperatures -20°C for at least 36 months.  Use as soon as possible after opening. Please avoid freeze-thaw cycles.

Date de péremption: 36 months

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: Epidermal growth factor

Sujet: Human epidermal growth factor, also known as EGF, is a 6.2kD protein with 53 amino acid residues and three intramolecular disulfide bonds. By binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface, EGF results in a variety of biochemical changes within the cell, rising intracellular calcium levels, increasing glycolysis, protein synthesis and regulating the gene expression, including the gene for EGFR that ultimately lead to DNA synthesis and cell proliferation.

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation